Separation of anions, developed in pioneering work that demonstrated FI-CE hyphenation as discussed in the previous slide (Karlberg & Kuban 2000), is “translated” here into LOV format, in order to demonstrate the feasibility and advantages of such modification. Using a standard LOV module, where the flow cell compartment is used to house the capillary and ground electrode (cathode), it was possible to automate sample delivery, sample pretreatment and electrolyte exchange and to accelerate the capillary washout. This work demonstrates that manipulation of sample, electrolytes and auxiliary reagents by combination of electric field and programmable flow, driven by pressure pumping, is a versatile tool for sample dilution, capillary reconditioning and automation of assay protocols. Using chromate- containing electrolyte for indirect spectrophotometric detection, a concentration range of 0.5-3.0 mM was assayed, while with sample stacking, sensitivity of anion assay increased 10-fold.
Anions by CE
2.4.14.
